celltrace cfse cell proliferation kit (Thermo Fisher)

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Thermo Fisher celltrace cfse cell proliferation kit
Celltrace Cfse Cell Proliferation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfse+celltrace+cfse+cell+proliferation+kit/celltrace+violet/pmc12139521-68-0-6
Average 90 stars, based on 1 article reviews

celltrace cfse cell proliferation kit - by Bioz Stars, 2026-10

90/100 stars

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Proliferation Assay:

Article Title: Solitomab, an EpCAM/CD3 bispecific antibody construct (BiTE®), is highly active against primary uterine serous papillary carcinoma cell lines in vitro
Article Snippet: .. Proliferation assay of tumor associated T-lymphocytes (TAL) after the addition of EpCAM BiTE® by CFSE Cell proliferation Briefly, cells were harvested from ascites samples and washed twice with PBS and immediately stained with carboxyfluorescein succinimidyl ester (CFSE) (CellTrace CFSE Cell Proliferation Kit, Invitrogen, Carlsbad, CA) at a working concentration of 10 micromolar. ..

Staining:

Concentration Assay:

Article Title: Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulations
Article Snippet: To generate apoptotic cells, Jurkat T cells, cultured in RPMI 1640 (Thermo Fisher, Cat# 11875–093) with 10% heat-inactivated FBS (Thermo Fisher, Cat# 10438025) at 37°C, 5% CO 2 10 mL were collected from a cell culture flask, washed with PBS, pelleted gently, and resuspended in 1 mL PBS. .. For labeling Jurkat cells, CFSE (CellTrace CFSE Cell Proliferation Kit, Thermo Fisher, Cat# C34554) was added to cells at final 5 μM concentration and incubated at 37°C for 20 min; 10 mL of RPMI 1640 (10% heat-inactivated FBS) was then added, mixed by vortexing, and further incubated at 37°C for 5 min. After washing, cells were resuspended in 1 mL serum-free RPMI 1640 medium. .. Apoptosis was induced by treatment with 1 μM staurosporine (Millipore Sigma, Cat# 19–123) for 5 hr at 37°C, 5% CO 2 , followed by washes in RPMI 1640 (10% heat-inactivated FBS) and resuspension in 1 mL RPMI 1640 (10% heat-inactivated FBS) for use in efferocytosis assays.

Article Title: Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulations
Article Snippet: To generate apoptotic cells, Jurkat T cells, cultured in RPMI 1640 (Thermo Fisher, Cat# 11875–093) with 10% heat- inactivated FBS (Thermo Fisher, Cat# 10438025) at 37°C, 5% CO2 10 mL were collected from a cell culture flask, washed with PBS, pelleted gently, and resuspended in 1 mL PBS. .. For labeling Jurkat cells, CFSE (CellTrace CFSE Cell Proliferation Kit, Thermo Fisher, Cat# C34554) was added to cells at final 5 μM concentration and incubated at 37°C for 20 min; 10 mL of RPMI 1640 (10% heat- inactivated FBS) was then added, mixed by vortexing, and further incubated at 37°C for 5 min. After washing, cells were resuspended in 1 mL serum- free RPMI 1640 medium. .. Apoptosis was induced by treatment with 1 μM staurosporine (Millipore Sigma, Cat# 19–123) for 5 hr at 37°C, 5% CO2, followed by washes in RPMI 1640 (10% heat- inactivated FBS) and resuspension in 1 mL RPMI 1640 (10% heat- inactivated FBS) for use in efferocytosis assays.

other:

Article Title: CD73 blockade promotes dendritic cell infiltration of irradiated tumors and tumor rejection
Article Snippet: On day 14–16 of culture, cells were analyzed by flow cytometry for cDC1 percentage, which was consistently >80%, and labeled with carboxyfluorescein (CFSE) (CellTraceTM CFSE Cell Proliferation Kit, Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"C34554","term_id":"2370695","term_text":"C34554"}} C34554 ).

Article Title: Immune Effects of Cryoablation in Woodchuck Hepatocellular Carcinoma
Article Snippet: PBMC (1*10 6 /mL in PBS) were supplemented with 1.2 μM carboxyfluorescein succinimidyl ester (CFSE) (CellTraceTM CFSE Cell Proliferation Kit, Invitrogen, cat# C34554).

Labeling:

Article Title: Modeling the Pathological Long-Range Regulatory Effects of Human Structural Variation with Patient-Specific hiPSCs.
Article Snippet: .. 5x106 WT or BOFS p2hNCC were labeled with 10 mM Carboxyfluorescein succinimidyl ester (CFSE) (CellTrace CFSE Cell Proliferation Kit; MolecularProbes) in 1ml PBS or in PBS without dye and incubated for 10 min at 37 C. The reaction was quenched by addition of 5 mL ice-cold hNCC maintenance medium followed by a 5 min incubation on ice. ..

Article Title: Lsd1 safeguards T-cell development via suppressing endogenous retroelements and interferon responses.
Article Snippet: .. Briefly, a single-cell suspension of splenocytes was prepared and labeled with CFSE (CellTrace CFSE Cell Proliferation Kit; Thermo Fisher Scientific). ..

Article Title: Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulations
Article Snippet: To generate apoptotic cells, Jurkat T cells, cultured in RPMI 1640 (Thermo Fisher, Cat# 11875–093) with 10% heat- inactivated FBS (Thermo Fisher, Cat# 10438025) at 37°C, 5% CO2 10 mL were collected from a cell culture flask, washed with PBS, pelleted gently, and resuspended in 1 mL PBS. .. For labeling Jurkat cells, CFSE (CellTrace CFSE Cell Proliferation Kit, Thermo Fisher, Cat# C34554) was added to cells at final 5 μM concentration and incubated at 37°C for 20 min; 10 mL of RPMI 1640 (10% heat- inactivated FBS) was then added, mixed by vortexing, and further incubated at 37°C for 5 min. After washing, cells were resuspended in 1 mL serum- free RPMI 1640 medium. .. Apoptosis was induced by treatment with 1 μM staurosporine (Millipore Sigma, Cat# 19–123) for 5 hr at 37°C, 5% CO2, followed by washes in RPMI 1640 (10% heat- inactivated FBS) and resuspension in 1 mL RPMI 1640 (10% heat- inactivated FBS) for use in efferocytosis assays.

Incubation:

Article Title: Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulations
Article Snippet: To generate apoptotic cells, Jurkat T cells, cultured in RPMI 1640 (Thermo Fisher, Cat# 11875–093) with 10% heat- inactivated FBS (Thermo Fisher, Cat# 10438025) at 37°C, 5% CO2 10 mL were collected from a cell culture flask, washed with PBS, pelleted gently, and resuspended in 1 mL PBS. .. For labeling Jurkat cells, CFSE (CellTrace CFSE Cell Proliferation Kit, Thermo Fisher, Cat# C34554) was added to cells at final 5 μM concentration and incubated at 37°C for 20 min; 10 mL of RPMI 1640 (10% heat- inactivated FBS) was then added, mixed by vortexing, and further incubated at 37°C for 5 min. After washing, cells were resuspended in 1 mL serum- free RPMI 1640 medium. .. Apoptosis was induced by treatment with 1 μM staurosporine (Millipore Sigma, Cat# 19–123) for 5 hr at 37°C, 5% CO2, followed by washes in RPMI 1640 (10% heat- inactivated FBS) and resuspension in 1 mL RPMI 1640 (10% heat- inactivated FBS) for use in efferocytosis assays.

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celltrace cfse cell proliferation kit (Thermo Fisher)

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